Microbial production of 4-thiouridine.

Weare reporting an efficient method for microbial production of 4-thiouridine as an alternative to chemical synthesis. A loopful of a 10-day slant culture of S. libani NK-1 10134 grown on yeast extractstarch agar was inoculated into a 500-ml Erlenmeyer flask containing 1 10 ml ofa seed mediumcomposed of soluble starch 2.0%, glucose 0.5%, peptone 0.5%, yeast extract 0.5%, soybeanmeal0.5%, K2HPO4 0.05%, MgSO4 0.05% and CaCO3 0.2% (pH 7.2) prepared in tap water. After a 2-day incubation on a rotary shaker (190rpm, 27°C), 2ml of the seed culture were